| EVENT MILES | STAGE MILES | SEGMENT MILES | DIRECTION | |
| 0.0 | 0.0 | 0.0 | Start | Grayson Highlands State Park Visitors Center |
| 4.0 | 4.0 | 4.0 | S | Grayson Highlands State Park Office and Gate |
| 4.8 | 4.8 | 0.8 | R | Highlands Parkway #58 (stop sign at Park Entrance) |
| 8.2 | 8.2 | 3.4 | L | Sturgill Rd. (converts to Helton Creek at VA/NC line) |
| 11.7 | 11.7 | 3.5 | R | 194 South (stop sign, to Lansing and West Jefferson) |
| 16.5 | 16.5 | 4.8 | L | 194 South (red light in Lansing) |
| 18.4 | 18.4 | 1.9 | R | Pounding Mill Rd. # 1349 |
| 19.5 | 19.5 | 1.1 | L | Teaberry #1347 |
| 21.6 | 21.6 | 2.1 | L | Staggs Creek #1342 (stop sign) |
| 21.8 | 21.8 | 0.2 | R | 88 West (stop sign) |
| 24.8 | 24.8 | 3.0 | | Clifton Methodist Church/Ashe Outreach Ministries (right) |
